Publications (2007 - present)

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2017

Wang YA, Wu D, Auclair JR, Salisbury JP, Sarin R, Tang Y, Mozdzierz NJ, Shah K, Zhang AF, Wu SL, Agar JN, Love JC, Love KR, Hancock WS. Integrated bottom-up and top-down liquid chromatography-mass spectrometry (LC-MS) for characterization of recombinant human growth hormone degradation productsAnal Chem. 2017 Nov 3. doi: 10.1021/acs.analchem.7b03026.

Jia B, McNeil LK, Dupont CD, Tsioris K, Barry RM, Scully IL, Ogunniyi AO, Gonzalez C, Pride MW, Gierahn TM, Liberator PA, Jansen KU, Love JC. Longitudinal multiparameter single-cell analysis of macaques immunized with pneumococcal protein-conjugated or unconjugated polysaccharide vaccines reveals distinct antigen specific memory B cell repertoires. PLoS One. 2017 Sep 14;12(9):e0183738. doi: 10.1371/journal.pone.0183738.

Goods BA, Hernandez AL, Lowther DE, Lucca LE, Lerner BA, Gunel M, Raddassi K, Coric V, Hafler DA, Love JC. Functional differences between PD-1+ and PD-1- CD4+ effector T cells in healthy donors and patients with glioblastoma multiforme. PLoS One. 2017 Sep 7;12(9):e0181538. doi: 10.1371/journal.pone.0181538.

Matthews CB, Kuo A, Love KR, Love JC. Development of a general defined medium for Pichia pastorisBiotechnol Bioeng. 2017 Sep 2. doi: 10.1002/bit.26440.

Matthews CB, Wright C, Kuo A, Colant N, Westoby M, Love JC. Reexamining opportunities for therapeutic protein production in eukaryotic microorganisms. Biotechnol Bioeng. 2017 Nov;114(11):2432-2444. doi: 10.1002/bit.26378.

Severson KA, Monian B, Love JC, Braatz RD. A method for learning a sparse classifier in the presence of missing data for high-dimensional biological datasets. Bioinformatics. 2017 Sep 15;33(18):2897-2905. doi: 10.1093/bioinformatics/btx224.

Gierahn TM, Wadsworth MH 2nd, Hughes TK, Bryson BD, Butler A, Satija R, Fortune S, Love JC, Shalek AK. Seq-Well: portable, low-cost RNA sequencing of single cells at high throughputNat Methods. 2017 Feb 13. doi: 10.1038/nmeth.4179.

Taylor RA, Chang CF, Goods BA, Hammond MD, Mac Grory B, Ai Y, Steinschneider AF, Renfroe SC, Askenase MH, McCullough LD, Kasner SE, Mullen MT, Hafler DA, Love JC, Sansing LH. TGF-β1 modulates microglial phenotype and promotes recovery after intracerebral hemorrhageJ Clin Invest. 2017 Jan 3;127(1):280-292. doi: 10.1172/JCI88647.

2016

Huang Y, Ferrari G, Alter G, Forthal DN, Kappes JC, Lewis GK, Love JC, Borate B, Harris L, Greene K, Gao H, Phan TB, Landucci G, Goods BA, Dowell KG, Cheng HD, Bailey-Kellogg C, Montefiori DC, Ackerman ME. Diversity of Antiviral IgG Effector Activities Observed in HIV-Infected and Vaccinated SubjectsJ Immunol. 2016 Dec 15;197(12):4603-4612.

Chen X, Love JC, Navin NE, Pachter L, Stubbington MJ, Svensson V, Sweedler JV, Teichmann SA. Single-cell analysis at the thresholdNat Biotechnol. 2016 Nov 8;34(11):1111-1118. doi: 10.1038/nbt.3721.

Saung MT, Sharei A, Adalsteinsson VA, Cho N, Kamath T, Ruiz C, Kirkpatrick J, Patel N, Mino-Kenudson M, Thayer SP, Langer R, Jensen KF, Liss AS, Love JC. A Size-Selective Intracellular Delivery PlatformSmall. 2016 Sep 4. doi: 10.1002/smll.201601155.

Love KR, Shah KA, Whittaker CA, Wu J, Bartlett MC, Ma D, Leeson RL, Priest M, Borowsky J, Young SK, Love JC. Comparative genomics and transcriptomics of Pichia pastorisBMC Genomics. 2016 Aug 5;17:550. doi: 10.1186/s12864-016-2876-y. Erratum in: BMC Genomics. 2016;17(1):762.

Donius LR, Cheng Y, Choi J, Sun ZY, Hanson M, Zhang M, Gierahn TM, Marquez S, Uduman M, Kleinstein SH, Irvine D, Love JC, Reinherz EL, Kim M. Generation of Long-Lived Bone Marrow Plasma Cells Secreting Antibodies Specific for the HIV-1 gp41 Membrane-Proximal External Region in the Absence of PolyreactivityJ Virol. 2016 Sep 12;90(19):8875-90. doi: 10.1128/JVI.01089-16.

Lowther DE, Goods BA, Lucca LE, Lerner BA, Raddassi K, van Dijk D, Hernandez AL, Duan X, Gunel M, Coric V, Krishnaswamy S, Love JC, Hafler DA. PD-1 marks dysfunctional regulatory T cells in malignant gliomasJCI Insight. 2016 Apr 21;1(5). pii: e85935.

Liao MC, Muratore CR, Gierahn TM, Sullivan SE, Srikanth P, De Jager PL, Love JC, Young-Pearse TL. Single-Cell Detection of Secreted Aβ and sAPPα from Human IPSC-Derived Neurons and AstrocytesJ Neurosci. 2016 Feb 3;36(5):1730-46. doi: 10.1523/JNEUROSCI.2735-15.2016.

2015

Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencingTsioris K, Gupta NT, Ogunniyi AO, Zimnisky RM, Qian F, Yao Y, Wang X, Stern JN, Chari R, Briggs AW, Clouser CR, Vigneault F, Church GM, Garcia MN, Murray KO, Montgomery RR, Kleinstein SH, Love JC. Integr Biol (Camb). 2015 Dec;7(12):1587-97. doi: 10.1039/c5ib00169b.

Tsioris K, Gupta NT, Ogunniyi AO, Zimnisky RM, Qian F, Yao Y, Wang X, Stern JN, Chari R, Briggs AW, Clouser CR, Vigneault F, Church GM, Garcia MN, Murray KO, Montgomery RR, Kleinstein SH, Love JC. Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing. Integr Biol (Camb) 2015. 2015 Dec;7(12):1587-97. doi: 10.1039/c5ib00169b.

Patil SU, Ogunniyi AO, Calatroni A, Tadigotla VR, Ruiter B, Ma A, Moon J, Love JC, Shreffler WG. Peanut oral immunotherapy transiently expands circulating Ara h2-specific B cells with a homologous repertoire in unrelated subjects. J Allergy Clin Immunol. 2015 Jul;136(1):125-134.e12. doi: 10.1016/j.jaci.2015.03.026.

Mozdzierz NJ, Love KR, Lee KS, Lee HL, Shah KA, Ram RJ, Love JC. A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production. Lab Chip. 2015 Jun 30;15(14):2918-22. doi: 10.1039/c5lc00443h

Ozkumur AY, Goods BA, Love JC. Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning. Small. 2015 Jun 29. doi: 10.1002/smll.201500674.

Shah KA, Clark JJ, Goods BA, Politano TJ, Mozdzierz NJ, Zimnisky RM, Leeson RL, Love JC, Love KR. Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris. Biotechnol Bioeng. 2015 Jun 1. doi: 10.1002/bit.25663.

Cao Y, Goods BA, Raddassi K, Nepom GT, Kwok WW, Love JC, Hafler DA. Functional inflammatory profiles distinguish myelin-reactive T cells from patients with
multiple sclerosis
. Sci Transl Med. 2015 May 13;7(287):287ra74. doi: 10.1126/scitranslmed.aaa8038.

Zhang CZ, Adalsteinsson VA, Francis J, Cornils H, Jung J, Maire C, Ligon KL, Meyerson M, Love JC. Calibrating genomic and allelic coverage bias in single-cell
sequencing
. Nat Commun. 2015 Apr 16;6:6822. doi: 10.1038/ncomms7822.

2014

Torres AJ, Hill AS, Love JC. Nanowell-based immunoassays for measuring single-cell secretion: characterization of transport and surface binding. Anal Chem. 2014 Dec 2;86(23):11562-9. doi: 10.1021/ac4030297. Epub 2014 Nov 17.

Yao X, Williamson C, Adalsteinsson VA, D'Agostino RS, Fitton T, Smaroff GG, William RT, Wittrup KD, Love JC. Tumor cells are dislodged into the pulmonary vein during lobectomy. J Thorac Cardiovasc Surg. 2014 Dec;148(6):3224-3231.e5. doi: 10.1016/j.jtcvs.2014.06.074. Epub 2014 Jul 23.

Castellarnau M, Szeto GL, Su HW, Tokatlian T, Love JC, Irvine DJ, Voldman J. Stochastic Particle Barcoding for Single-Cell Tracking and Multiparametric Analysis. Small. 2014 Sep 2. doi: 10.1002/smll.201401369. 

Tsioris K, Torres AJ, Douce TB, Love JC. A new toolbox for assessing single cells. Annu Rev Chem Biomol Eng. 2014;5:455-77. doi: 10.1146/annurev-chembioeng-060713-035958.

Francis JM, Zhang CZ, Maire CL, Jung J, Manzo VE, Adalsteinsson VA, Homer H, Haidar S, Blumenstiel B, Pedamallu CS, Ligon AH, Love JC, Meyerson M, Ligon KL. EGFR variant heterogeneity in glioblastoma resolved through single-nucleus sequencing. Cancer Discov. 2014 Jun 3. pii: CD-13-0879.

Adalsteinsson VA, Love JC. Towards Engineered Processes for Sequencing-Based Analysis of Single Circulating Tumor Cells. Curr Opin Chem Eng. 2014 May 1;4:97-104.

Lohr JG, Adalsteinsson VA, Cibulskis K, Choudhury AD, Rosenberg M, Cruz-Gordillo P, Francis JM, Zhang CZ, Shalek AK, Satija R, Trombetta JJ, Lu D, Tallapragada N, Tahirova N, Kim S, Blumenstiel B, Sougnez C, Lowe A, Wong B, Auclair D, Van Allen EM, Nakabayashi M, Lis RT, Lee GS, Li T, Chabot MS, Ly A, Taplin ME, Clancy TE, Loda M, Regev A, Meyerson M, Hahn WC, Kantoff PW, Golub TR, Getz G, Boehm JS, Love JC. Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer. Nat Biotechnol. 2014 May;32(5):479-84. doi: 10.1038/nbt.2892. Epub 2014 Apr 20.

Ogunniyi AO, Thomas BA, Politano TJ, Varadarajan N, Landais E, Poignard P, Walker BD,Kwon DS, Love JC. Profiling human antibody responses by integrated single-cell analysis. Vaccine. 2014 May 19;32(24):2866-73. doi: 10.1016/j.vaccine.2014.02.020. Epub 2014 Mar 3

Yao X, Choudhury AD, Yamanaka YJ, Adalsteinsson VA, Gierahn TM, Williamson CA, Lamb CR, Taplin ME, Nakabayashi M, Chabot MS, Li T, Lee GS, Boehm JS, Kantoff PW, Hahn WC, Wittrup KD, Love JC. Functional analysis of single cells identifies a rare subset of circulating tumor cells with malignant traits. Integr Biol (Camb). 2014 Apr;6(4):388-98. doi: 10.1039/c3ib40264a. Epub 2014 Feb 13.

Zhou P, Shaffer DR, Alvarez Arias DA, Nakazaki Y, Pos W, Torres AJ, Cremasco V, Dougan SK, Cowley GS, Elpek K, Brogdon J, Lamb J, Turley SJ, Ploegh HL, Root DE, Love JC, Dranoff G, Hacohen N, Cantor H, Wucherpfennig KW. In vivo discovery of immunotherapy targets in the tumour microenvironment. Nature. 2014 Feb 6;506(7486):52-7. doi: 10.1038/nature12988. Epub 2014 Jan 29.

Gierahn TM, Loginov D, Love JC. Crossword: a fully automated algorithm for the segmentation and quality control of protein microarray images. J Proteome Res. 2014 Feb 7;13(2):362-71. doi: 10.1021/pr401167h. Epub 2014 Jan 24.

 

2013

Adalsteinsson, V. A., Tahirova, N., Tallapragada, N., Yao, X., Campion, L., Angelini, A., Douce, T. B., Huang, C., Bowman, B., Williamson, C. A., Kwon, D. S., Wittrup, K. D. & Love, J. C. Single cells from human primary colorectal tumors exhibit polyfunctional heterogeneity in secretions of ELR+ CXC chemokines. Integr Biol (Camb) 5, 1272-1281, doi:10.1039/c3ib40059j (2013).

Reuel, N. F., Grassbaugh, B., Kruss, S., Mundy, J. Z., Opel, C., Ogunniyi, A. O., Egodage, K., Wahl, R., Helk, B., Zhang, J., Kalcioglu, Z. I., Tvrdy, K., Bellisario, D. O., Mu, B., Blake, S. S., Van Vliet, K. J., Love, J. C., Wittrup, K. D. & Strano, M. S. Emergent Properties of Nanosensor Arrays: Applications for Monitoring IgG Affinity Distributions, Weakly Affined Hypermannosylation, and Colony Selection for Biomanufacturing. ACS Nano, doi:10.1021/nn403215e (2013).

Nguyen, C. Q., Ogunniyi, A. O., Karabiyik, A. & Love, J. C. Single-cell analysis reveals isotype-specific autoreactive B cell repertoires in Sjogren's syndrome. PLoS One 8, e58127, doi:10.1371/journal.pone.0058127 (2013).

Love, K. R., Bagh, S., Choi, J. & Love, J. C. Microtools for single-cell analysis in biopharmaceutical development and manufacturing. Trends Biotechnol 31, 280-286, doi:10.1016/j.tibtech.2013.03.001 (2013).

Torres, A. J., Contento, R. L., Gordo, S., Wucherpfennig, K. W. & Love, J. C. Functional single-cell analysis of T-cell activation by supported lipid bilayer-tethered ligands on arrays of nanowells. Lab Chip 13, 90-99, doi:10.1039/c2lc40869d (2013).

Love, J. C., Love, K. R. & Barone, P. W. Enabling Global Access to High-Quality Biopharmaceuticals. Current Opinion in Chemical Engineering (2013).

2012

Yamanaka, Y. J., Szeto, G. L., Gierahn, T. M., Forcier, T. L., Benedict, K. F., Brefo, M. S., Lauffenburger, D. A., Irvine, D. J. & Love, J. C. Cellular barcodes for efficiently profiling single-cell secretory responses by microengraving. Anal Chem 84, 10531-10536, doi:10.1021/ac302264q (2012).

Yamanaka, Y. J., Gierahn, T. M. & Love, J. C. The dynamic lives of T cells: new approaches and themes. Trends Immunol., doi:10.1016/j.it.2012.10.006 (2012).

Yamanaka, Y. J., Berger, C. T., Sips, M., Cheney, P. C., Alter, G. & Love, J. C. Single-cell analysis of the dynamics and functional outcomes of interactions between human natural killer cells and target cells. Integr Biol (Camb) 4, 1175-1184, doi:10.1039/c2ib20167d (2012).

Yalcin, A., Yamanaka, Y. J. & Love, J. C. Analytical technologies for integrated single-cell analysis of human immune responses. Methods Mol Biol 853, 211-235, doi:10.1007/978-1-61779-567-1_16 (2012).

Love, K. R., Politano, T. J., Panagiotou, V., Jiang, B., Stadheim, T. A. & Love, J. C. Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity. PLoS One 7, e37915, doi:10.1371/journal.pone.0037915 (2012).

Varadarajan, N., Kwon, D. S., Law, K. M., Ogunniyi, A. O., Anahtar, M. N., Richter, J. M., Walker, B. D. & Love, J. C. Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving. Proc. Natl. Acad. Sci. USA (2012).

Han, Q., Bagheri, N., Bradshaw, E. M., Hafler, D. A., Lauffenburger, D. A. & Love, J. C. Polyfunctional responses by human T cells result from sequential release of cytokines. Proc. Natl. Acad. Sci. USA 109, 1605-1612, doi:10.1073/pnas.1117194109 (2012).
(See also accompanying commentary:  Haining, W.N., Travels in time:  Assessing the functional complexity of T cells. Proc. Natl. Acad. Sci. USA 109, 1359-1360, doi:10.1073/pnas.1119856109 (2012).)

 

2011

Varadarajan, N., Julg, B., Yamanaka, Y. J., Chen, H., Ogunniyi, A. O., McAndrew, E., Porter, L. C., Piechocka-Trocha, A., Hill, B. J., Douek, D. C., Pereyra, F., Walker, B. D. & Love, J. C. A high-throughput single-cell analysis of human CD8 T cell functions reveals discordance for cytokine secretion and cytolysis. J. Clin. Invest. 121, 4322-4331, doi:10.1172/JCI58653 (2011).

Zhao, W., Schafer, S., Choi, J., Yamanaka, Y. J., Lombardi, M. L., Bose, S., Carlson, A. L., Phillips, J. A., Teo, W., Droujinine, I. A., Cui, C. H., Jain, R. K., Lammerding, J., Love, J. C., Lin, C. P., Sarkar, D., Karnik, R. & Karp, J. M. Cell-surface sensors for real-time probing of cellular environments. Nat. Nanotechnol. 6, 524-531, doi:10.1038/nnano.2011.101 (2011).

Choi, J., Love, K. R., Gong, Y., Gierahn, T. M. & Love, J. C. Immuno-hybridization chain reaction for enhancing detection of individual cytokine-secreting human peripheral mononuclear cells. Anal. Chem. 83, 6890-6895, doi:10.1021/ac2013916 (2011).

Panagiotou, V., Love, K. R., Jiang, B., Nett, J., Stadheim, T. & Love, J. C. Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving. Appl. Environ. Microb. 77, 3154-3156, doi:10.1128/AEM.00104-11 (2011).

 

2010

Love, J. C. Making antibodies from scratch. Nat. Biotechnol. 28, 1176-1178, doi:10.1038/nbt1110-1176 (2010).

Love, J.C. Integrated process design for single-cell analytical technologies. AIChE J. 56, 2496-2502 (2010).

Gong, Y., Ogunniyi, A.O. & Love, J.C. Massively parallel detection of gene expression in single cells using subnanoliter wells. Lab Chip 10, 2334-2337 (2010).

Han, Q., Bradshaw, E.M., Nilsson, B., Hafler, D.A. & Love, J.C. Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving. Lab Chip 10, 1391-1400 (2010). 

Love, K.R., Panagiotou, V., Jiang, B., Stadheim, T.A. & Love, J.C. Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. Biotechnol. Bioeng. 106, 319-325 (2010).

Choi, J.H., Ogunniyi, A.O., Du, M., Du, M., Kretschmann, M., Eberhardt, J. & Love, J.C. Development and optimization of a process for automated recovery of single cells identified by microengraving. Biotechnol. Prog. 26, 888-895 (2010).

Schmidt, D.J., Pridgen, E.M., Hammond, P.T. & Love, J.C. Layer-by-layer assembly of a pH-sensitive and electrochromic thin film. J. Chem. Educ. 87, 208-211 (2010).

Song, Q., Han, Q., Bradshaw, E.M., Kent, S.C., Raddassi, K., Nilsson, B., Nepom, G.T., Hafler, D.A. & Love, J.C. On-chip activation and subsequent detection of individual antigen-specific T cells. Anal. Chem. 82, 473-477 (2010).

 

2009

Ogunniyi, A.O., Story, C.M., Papa, E., Guillen, E. & Love, J.C. Screening individual hybridomas by microengraving to discover monoclonal antibodies. Nature Protocols 4, 767-782 (2009).

Hu, C.-C.A., Dougan, S.K., McGehee, A.M., Love, J.C. & Ploegh, H.L. XBP-1 regulates signal transduction, transcription factors and bone marrow colonization in B cells. EMBO J. 28, 1624-1636 (2009).

Ronan, J.L., Story, C.M., Papa, E. & Love, J.C. Optimization of the surfaces used to capture antibodies from single hybridomas reduces the time required for microengraving. J. Immunol. Methods 340, 164-169 (2009).

 

2008

Story, C.M., Papa, E., Hu, C.-C.A., Ronan, J.L., Herlihy, K., Ploegh, H.L. & Love, J.C. Profiling antibody responses by multiparametric analysis of single B cells. Proc. Natl. Acad. Sci. U. S. A. 105, 17902-17907 (2008).

Bradshaw, E. M., Kent, S. C., Tripuraneni, V., Orban, T., Ploegh, H. L., Hafler, D. A. & Love, J. C. Concurrent detection of secreted products from human lymphocytes by microengraving: cytokines and antigen-reactive antibodies. Clin Immunol 129, 10-18, doi:10.1016/j.clim.2008.06.009 (2008).

Wong, A. P., Castillejos, R. P., Love, J. C. & Whitesides, G. M. Partitioning Microfluidic Channels with Hydrogel to Construct Tunable 3-D Cellular Microenvironments. Biomaterials 29, 1853-1861 (2008).

 

2007

Whitesides, G. M. & Love, J. C. The Art of Building Small. Scientific American Reports (2007).

Leduc, P. R., Wong, M. S., Ferreira, P. M., Groff, R. E., Haslinger, K., Koonce, M. P., Lee, W. Y., Love, J. C., McCammon, J. A., Monteiro-Riviere, N. A., Rotello, V. M., Rubloff, G. W., Westervelt, R. & Yoda, M. Towards an in vivo biologically inspired nanofactory. Nature Nanotechnology 2, 3-7 (2007).

Vyas, J. M., Kim, Y.-M., Artavanis-Tsakonas, K., Love, J. C., van der Veen, A. G. & Ploegh, H. L. Tubulation of Class II MHC compartments is microtubule-dependent and involves multiple endolysosomal membrane proteins in primary dendritic cells. J. Immunol. 178, 7199-7210 (2007).

Leduc, P. R., Wong, M. S., Ferreira, P. M., Groff, R. E., Haslinger, K., Koonce, M. P., Lee, W. Y., Love, J. C., McCammon, J. A., Monteiro-Riviere, N. A., Rotello, V. M., Rubloff, G. W., Westervelt, R. & Yoda, M. Towards an in vivo biologically inspired nanofactory. Nature Nanotechnology 2, 3-7 (2007).