Utilizing our developed technologies like Seq-Well, the lab has extensive experience with genomic profiling of immune cells in the context of cancer, allergy, and vaccine responses. For example, in collaboration with the Spranger lab, we have characterized mechanisms of T cell dysfunction in lung cancer and how immunotherapies such as checkpoint blockade can overcome resistance. Our work often profiles T cell receptors with the transcriptome to uncover clonotype-phenotype relationships.
Relevant Publications
Cancer
Morgan DM, Horton BL, Bhandarkar V, Van R, Dinter T, Zagorulya M, Love JC, Spranger S. Expansion of tumor-reactive CD8+ T cell clonotypes occurs in the spleen in response to immune checkpoint blockade. Sci Immunol. 2024 Sep 13;9(99):eadi3487. doi: 10.1126/sciimmunol.adi3487. Epub 2024 Sep 13. PMID: 39270006.
Zagorulya M, Yim L, Morgan DM, Edwards A, Torres-Mejia E, Momin N, McCreery CV, Zamora IL, Horton BL, Fox JG, Wittrup KD, Love JC, Spranger S. Tissue-specific abundance of interferon-gamma drives regulatory T cells to restrain DC1-mediated priming of cytotoxic T cells against lung cancer. Immunity. 2023 Feb 14;56(2):386-405.e10. doi: 10.1016/j.immuni.2023.01.010. Epub 2023 Feb 2. PMID: 36736322.
Horton BL, Morgan DM, Momin N, Zagorulya M, Torres-Mejia E, Bhandarkar V, Wittrup KD, Love JC, Spranger S. Lack of CD8+ T cell effector differentiation during priming mediates checkpoint blockade resistance in non-small cell lung cancer. Sci Immunol. 2021 Oct 29;6(64):eabi8800. doi: 10.1126/sciimmunol.abi8800. Epub 2021 Oct 29. PMID: 34714687; PMCID: PMC10786005.
Vaccine Responses
Rodrigues KA, Zhang YJ, Lam J, Aung A, Morgan DM, Romanov A, Maiorino L, Yousefpour P, Gibson G, Ozorowski G, Gregory JR, Amlashi P, Van R, Buckley M, Ward AB, Schief WR, Love JC, Irvine DJ. Vaccines combining slow release and follicle targeting of antigens increase germinal center B cell diversity and clonal expansion. Sci Transl Med. 2025 Jun 18;17(803):eadw7499. doi: 10.1126/scitranslmed.adw7499. Epub 2025 Jun 18. PMID: 40531969.
Morgan DM, Zhang YJ, Kim JH, Murillo M, Singh S, Loschko J, Surendran N, Sekulovic O, Feng E, Shi S, Irvine DJ, Patil SU, Kanevsky I, Chorro L, Christopher Love J. Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to pneumococcal vaccination. Commun Biol. 2024 Sep 28;7(1):1208. doi: 10.1038/s42003-024-06823-0. PMID: 39341987; PMCID: PMC11438910.
Ma L, Hostetler A, Morgan DM, Maiorino L, Sulkaj I, Whittaker CA, Neeser A, Pires IS, Yousefpour P, Gregory J, Qureshi K, Dye J, Abraham W, Suh H, Li N, Love JC, Irvine DJ. Vaccine-boosted CAR T crosstalk with host immunity to reject tumors with antigen heterogeneity. Cell. 2023 Jul 20;186(15):3148-3165.e20. doi: 10.1016/j.cell.2023.06.002. Epub 2023 Jul 5. PMID: 37413990; PMCID: PMC10372881.
Allergy
LaHood NA, Min J, Keswani T, Richardson CM, Amoako K, Zhou J, Marini-Rapoport O, Bernard H, Hazebrouck S, Shreffler WG, Love JC, Pomes A, Pedersen LC, Mueller GA, Patil SU. Immunotherapy-induced neutralizing antibodies disrupt allergen binding and sustain allergen tolerance in peanut allergy. J Clin Invest. 2023 Jan 17;133(2):e164501. doi: 10.1172/JCI164501. PMID: 36647835; PMCID: PMC9843057.
Monian B, Tu AA, Ruiter B, Morgan DM, Petrossian PM, Smith NP, Gierahn TM, Ginder JH, Shreffler WG, Love JC. Peanut oral immunotherapy differentially suppresses clonally distinct subsets of T helper cells. J Clin Invest. 2022 Jan 18;132(2):e150634. doi: 10.1172/JCI150634. PMID: 34813505; PMCID: PMC8759778.
Morgan DM, Ruiter B, Smith NP, Tu AA, Monian B, Stone BE, Virk-Hundal N, Yuan Q, Shreffler WG, Love JC. Clonally expanded, GPR15-expressing pathogenic effector TH2 cells are associated with eosinophilic esophagitis. Sci Immunol. 2021 Aug 13;6(62):eabi5586. doi: 10.1126/sciimmunol.abi5586. PMID: 34389613; PMCID: PMC8686696.