Publications (2007 - present)



Andrew M. Biedermann, Isabella R. Gengaro, Sergio A. Rodriguez-Aponte, Kerry R. Love,  J. Christopher Love. Modular development enables rapid design of media for alternative hosts. bioRxiv. 2021 Apr 15:2021.04.15.440035. doi: 10.1101/2021.04.30.442183.

Dalvie NC, Biedermann AM, Rodriguez-Aponte SA, Naranjo CA, Rao HD, Rajurkar MP, Lothe RR, Shaligram US, Johnston RS, Crowell LE, Castelino S, Tracey MK, Whittaker CA, Love JC. Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii. bioRxiv. 2021 Apr 15:2021.04.15.440035. doi: 10.1101/2021.04.15.440035.

Dalvie NC, Rodriguez-Aponte SA, Hartwell BL, Tostanoski LH, Biedermann AM, Crowell LE, Kaur K, Kumru O, Carter L, Yu J, Chang A, McMahan K, Courant T, Lebas C, Lemnios AA, Rodrigues KA, Silva M, Johnston RS, Naranjo CA, Tracey MK, Brady JR, Whittaker CA, Yun D, Kar S, Porto M, Lok M, Andersen H, Lewis MG, Love KR, Camp DL, Silverman JM, Kleanthous H, Joshi SB, Volkin DB, Dubois PM, Collin N, King NP, Barouch DH, Irvine DJ, Love JC. Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters. bioRxiv. 2021 Mar 4. doi: 10.1101/2021.03.03.433558.


Dalvie NC, Brady JR, Crowell LE, Tracey MK, Biedermann AM, Kaur K, Hickey JM, Kristensen DL 2nd, Bonnyman AD, Rodriguez-Aponte SA, Whittaker CA, Bok M, Vega C, Mukhopadhyay TK, Joshi SB, Volkin DB, Parreño V, Love KR, Love JC. Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus. Microb Cell Fact. 2021 May 1;20(1):94. doi: 10.1186/s12934-021-01583-6.

Askenase MH, Goods BA, Beatty HE, Steinschneider AF, Velazquez SE, Osherov A, Landreneau MJ, Carroll SL, Tran TB, Avram VS, Drake RS, Gatter GJ, Massey JA, Karuppagounder SS, Ratan RR, Matouk CC, Sheth KN, Ziai WC, Parry-Jones AR, Awad IA, Zuccarello M, Thompson RE, Dawson J, Hanley DF, Love JC, Shalek AK, Sansing LH; MISTIE III Consortium; ICHseq investigators; ICHseq Investigators MISTIE III Investigators. Longitudinal transcriptomics define the stages of myeloid activation in the living human brain after intracerebral hemorrhage. Sci Immunol. 2021 Feb 19;6(56):eabd6279. doi: 10.1126/sciimmunol.abd6279.

Chang CF, Goods BA, Askenase MH, Beatty HE, Osherov A, DeLong JH, Hammond MD, Massey J, Landreneau M, Love JC, Sansing LH. Divergent Functions of Tissue-Resident and Blood-Derived Macrophages in the Hemorrhagic BrainStroke. 2021 May;52(5):1798-1808. doi: 10.1161/STROKEAHA.120.032196.

Crowell LE, Rodriguez SA, Love KR, Cramer SM, Love JC. Rapid optimization of processes for the integrated purification of biopharmaceuticals. Biotechnol Bioeng. 2021 Mar 30. doi: 10.1002/bit.27767.

Brady JR, Love JC. Alternative hosts as the missing link for equitable therapeutic protein productionNat Biotechnol. 2021 Apr;39(4):404-407. doi: 10.1038/s41587-021-00884-w.

Goods BA, Askenase MH, Markarian E, Beatty HE, Drake RS, Fleming I, DeLong JH, Philip NH, Matouk CC, Awad IA, Zuccarello M, Hanley DF, Love JC, Shalek AK, Sansing LH; ICHseq Investigators. Leukocyte dynamics after intracerebral hemorrhage in a living patient reveal rapid adaptations to tissue milieu. JCI Insight. 2021 Mar 22;6(6):e145857. doi: 10.1172/jci.insight.145857.

Crowell LE, Goodwine C, Holt CS, Rocha L, Vega C, Rodriguez SA, Dalvie NC, Tracey MK, Puntel M, Wigdorovitz A, Parreño V, Love KR, Cramer SM, Love JC. Development of a platform process for the production and purification of single-domain antibodies. Biotechnol Bioeng. 2021 Feb 24. doi: 10.1002/bit.27724.

Lu AE, Maloney AJ, Dalvie NC, Brady JR, Love KR, Love JC, Braatz RD. Modeling of copy number variability in Pichia pastoris. Biotechnol Bioeng. 2021 Feb 2. doi: 10.1002/bit.27698.

Sawant N, Kaur K, Holland DA, Hickey JM, Agarwal S, Brady JR, Dalvie NC, Tracey MK, Velez-Suberbie ML, Morris SA, Jacob SI, Bracewell DG, Mukhopadhyay TK, Love KR, Love JC, Joshi SB, Volkin DB. Rapid Developability Assessments to Formulate Recombinant Protein Antigens as Stable, Low-Cost, Multi-Dose Vaccine Candidates: Case-Study With Non-Replicating Rotavirus (NRRV) Vaccine Antigens. J Pharm Sci. 2021 Mar. doi: 10.1016/j.xphs.2020.11.039.

Kaur K, Xiong J, Sawant N, Agarwal S, Hickey JM, Holland DA, Mukhopadhyay TK, Brady JR, Dalvie NC, Tracey MK, Love KR, Love JC, Weis DD, Joshi SB, Volkin DB. Mechanism of Thimerosal-Induced Structural Destabilization of a Recombinant Rotavirus P[4] Protein Antigen Formulated as a Multi-Dose Vaccine. J Pharm Sci. 2021 Mar. doi: 10.1016/j.xphs.2020.11.033.

Hong MS, Velez-Suberbie ML, Maloney AJ, Biedermann A, Love KR, Love JC, Mukhopadhyay TK, Braatz RD. Macroscopic modeling of bioreactors for recombinant protein producing Pichia pastoris in defined medium. Biotechnol Bioeng. 2021 Mar. doi: 10.1002/bit.27643.


Hughes TK, Wadsworth MH 2nd, Gierahn TM, Do T, Weiss D, Andrade PR, Ma F, de Andrade Silva BJ, Shao S, Tsoi LC, Ordovas-Montanes J, Gudjonsson JE, Modlin RL, Love JC, Shalek A. Second-Strand Synthesis-Based Massively Parallel scRNA-Seq Reveals Cellular States and Molecular Features of Human Inflammatory Skin Pathologies. Immunity. 2020 Oct.13. doi: 10.1016/j.immuni.2020.09.015.

Brady JR, Tan MC, Whittaker CA, Colant NA, Dalvie NC, Love KR, Love JCIdentifying Improved Sites for Heterologous Gene Integration Using ATAC-seq. ACS Synth Biol. 2020 Sept. 18. doi: 10.1021/acssynbio.0c00299.

Lu J, Zhu X, Shui JE, Xiong L, Gierahn T, Zhang C, Wood M, Hally S, Love JC, Li H, Crawford BC, Mou H, Lerou PHRho/SMAD/mTOR triple inhibition enables long-term expansion of human neonatal tracheal aspirate-derived airway basal cell-like cells. Pediatr Res. 2020 May 4. doi: 10.1038/s41390-020-0925-3.

Kazer SW, Aicher TP, Muema DM, Carroll SL, Ordovas-Montanes J, Miao VN, Tu AA, Ziegler CGK, Nyquist SK, Wong EB, Ismail N, Dong M, Moodley A, Berger B, Love JC, Dong KL, Leslie A, Ndhlovu ZM, Ndung'u T, Walker BD, Shalek AK. Integrated single-cell analysis of multicellular immune dynamics during hyperacute HIV-1 infection. Nat Med. 2020 April 26. doi: 10.1038/s41591-020-0799-2.

Parsons HA, Rhoades J, Reed SC, Gydush G, Ram P, Exman P, Xiong K, Lo CC, Li T, Fleharty M, Kirkner GJ, Rotem D, Cohen O, Yu F, Fitarelli-Kiehl M, Leong KW, Hughes ME, Rosenberg SM, Collins LC, Miller KD, Blumenstiel B, Trippa L, Cibulskis C, Neuberg DS, DeFelice M, Freeman SS, Lennon NJ, Wagle N, Ha G, Stover DG, Choudhury AD, Getz G, Winer EP, Meyerson M, Lin NU, Krop I, Love JC, Makrigiorgos GM, Partridge AH, Mayer EL, Golub TR, Adalsteinsson VASensitive Detection of Minimal Residual Disease in Patients Treated for Early-Stage Breast Cancer. Clin Cancer Res. 2020 Jun. 1. doi: 10.1158/1078-0432.CCR-19-3005.


Dalvie NC, Leal J, Whittaker CA, Yang Y, Brady JR, Love KR, Love JC. Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii. ACS Synth Biol. 2019 Dec 13. doi: 10.1021/acssynbio.9b00372.

Tu AA, Gierahn TM, Monian B, Morgan DM, Mehta NK, Ruiter B, Shreffler WG, Shalek AK, Love JC. TCR sequencing paired with massively parallel 3' RNA-seq reveals clonotypic T cell signatures. Nat Immunol. 2019 Dec;20(12):1692-1699. doi: 10.1038/s41590-019-0544-5.

Ruiter B, Smith NP, Monian B, Tu AA, Fleming E, Virkud YV, Patil SU, Whittaker CA, Love JC, Shreffler WG. Expansion of the CD4+ effector T-cell repertoire characterizes peanut-allergic patients with heightened clinical sensitivity. J Allergy Clin Immunol. 2019 Oct 22. pii: S0091-6749(19)31331-4. doi: 10.1016/j.jaci.2019.09.033.

Brady JR, Whittaker CA, Tan MC, Kristensen DL 2nd, Ma D, Dalvie NC, Love KR, Love JC. Comparative genome-scale analysis of Pichia pastoris variants informs selection of an optimal base strain. Biotechnol Bioeng. 2019 Oct 26. doi: 10.1002/bit.27209.

Menon M, Mohammadi S, Davila-Velderrain J, Goods BA, Cadwell TD, Xing Y, Stemmer-Rachamimov A, Shalek AK, Love JC, Kellis M, Hafler BP. Single-cell transcriptomic atlas of the human retina identifies cell types associated with age-related macular degeneration. Nat Commun. 2019 Oct 26. doi: 10.1038/s41467-019-12780-8.

Vecchiarello N, Timmick SM, Goodwine C, Crowell LE, Love KR, Love JC, Cramer SM. A combined screening and in silico strategy for the rapid design of integrated downstream processes for process and product-related impurity removal. Biotechnol Bioeng. 2019 Sep. doi: 10.1002/bit.27018. Epub 2019 Jun 29.

Jiang H, Horwitz AA, Wright C, Tai A, Znameroski EA, Tsegaye Y, Warbington H, Bower BS, Alves C, Co C, Jonnalagadda K, Platt D, Walter JM, Natarajan V, Ubersax JA, Cherry JR, Love JC. Challenging the workhorse: Comparative analysis of eukaryotic micro-organisms for expressing monoclonal antibodies. Biotechnol Bioeng. 2019 Jun. doi: 10.1002/bit.26951. Epub 2019 Mar 7.


Mandal A, Boopathy AV, Lam LKW, Moynihan KD, Welch ME, Bennett NR, Turvey ME, Thai N, Van JH, Love JC, Hammond PT, Irvine DJ. Cell and fluid sampling microneedle patches for monitoring skin-resident immunity. Sci Transl Med. 2018 Nov 14. doi: 10.1126/scitranslmed.aar2227.

Choudhury AD, Werner L, Francini E, Wei XX, Ha G, Freeman SS, Rhoades J, Reed SC, Gydush G, Rotem D, Lo C, Taplin ME, Harshman LC, Zhang Z, O'Connor EP, Stover DG, Parsons HA, Getz G, Meyerson M, Love JC, Hahn WC, Adalsteinsson VA. Tumor fraction in cell-free DNA as a biomarker in prostate cancer. JCI Insight. 2018 Nov 2. doi: 10.1172/jci.insight.122109.

Crowell LE, Lu AE, Love KR, Stockdale A, Timmick SM, Wu D, Wang YA, Doherty W, Bonnyman A, Vecchiarello N, Goodwine C, Bradbury L, Brady JR, Clark JJ, Colant NA, Cvetkovic A, Dalvie NC, Liu D, Liu Y, Mascarenhas CA, Matthews CB, Mozdzierz NJ, Shah KA, Wu SL, Hancock WS, Braatz RD, Cramer SM, Love JC. On-demand manufacturing of clinical-quality biopharmaceuticals. Nat Biotechnol. 2018 Oct 1. doi: 10.1038/nbt.4262.

Dupont CD, Scully IL, Zimnisky RM, Monian B, Rossitto CP, O'Connell EB, Jansen KU, Anderson AS, Love JC. Two Vaccines for Staphylococcus aureus Induce a B-Cell-Mediated Immune Response. mSphere. 2018 Aug 22;3(4). pii: e00217-18. doi: 10.1128/mSphere.00217-18.

Viswanathan SR, Ha G, Hoff AM, Wala JA, Carrot-Zhang J, Whelan CW, Haradhvala NJ, Freeman SS, Reed SC, Rhoades J, Polak P, Cipicchio M, Wankowicz SA, Wong A, Kamath T, Zhang Z, Gydush GJ, Rotem D; PCF/SU2C International Prostate Cancer Dream Team, Love JC, Getz G, Gabriel S, Zhang CZ, Dehm SM, Nelson PS, Van Allen EM, Choudhury AD, Adalsteinsson VA, Beroukhim R, Taplin ME, Meyerson M. Structural Alterations Driving Castration-Resistant Prostate Cancer Revealed by Linked-Read Genome Sequencing. Cell. 2018 Jul 12;174(2):433-447.e19. doi: 10.1016/j.cell.2018.05.036.

Timmick SM, Vecchiarello N, Goodwine C, Crowell LE, Love KR, Love JC, Cramer SM. An impurity characterization based approach for the rapid development of integrated downstream purification processes. Biotechnol Bioeng. 2018 Aug;115(8):2048-2060. doi: 10.1002/bit.26718. 

Stover DG, Parsons HA, Ha G, Freeman SS, Barry WT, Guo H, Choudhury AD, Gydush G, Reed SC, Rhoades J, Rotem D, Hughes ME, Dillon DA, Partridge AH, Wagle N, Krop IE, Getz G, Golub TR, Love JC, Winer EP, Tolaney SM, Lin NU, Adalsteinsson VA. Association of Cell-Free DNA Tumor Fraction and Somatic Copy Number Alterations With Survival in Metastatic Triple-Negative Breast Cancer. J Clin Oncol. 2018 Feb 20;36(6):543-553. doi: 10.1200/JCO.2017.76.0033. 


Love KR, Dalvie NC, Love JC. The yeast stands alone: the future of protein biologic productionCurr Opin Biotechnol. 2017 Dec 22;53:50-58. doi: 10.1016/j.copbio.2017.12.010. 

Chang CF, Goods BA, Askenase MH, Hammond MD, Renfroe SC, Steinschneider AF, Landreneau MJ, Ai Y, Beatty HE, da Costa LHA, Mack M, Sheth KN, Greer DM, Huttner A, Coman D, Hyder F, Ghosh S, Rothlin CV, Love JC, Sansing LH. Erythrocyte efferocytosis modulates macrophages towards recovery after intracerebral hemorrhage. J Clin Invest. 2018 Feb 1;128(2):607-624. doi: 10.1172/JCI95612. 

Muratore CR, Zhou C, Liao M, Fernandez MA, Taylor WM, Lagomarsino VN, Pearse RV 2nd, Rice HC, Negri JM, He A, Srikanth P, Callahan DG, Shin T, Zhou M, Bennett DA, Noggle S, Love JC, Selkoe DJ, Young-Pearse TL. Cell-type Dependent Alzheimer's Disease Phenotypes: Probing the Biology of Selective Neuronal Vulnerability. Stem Cell Reports. 2017 Dec 12;9(6):1868-1884. doi: 10.1016/j.stemcr.2017.10.015.

Adalsteinsson VA, Ha G, Freeman SS, Choudhury AD, Stover DG, Parsons HA, Gydush G, Reed SC, Rotem D, Rhoades J, Loginov D, Livitz D, Rosebrock D, Leshchiner I, Kim J, Stewart C, Rosenberg M, Francis JM, Zhang CZ, Cohen O, Oh C, Ding H, Polak P, Lloyd M, Mahmud S, Helvie K, Merrill MS, Santiago RA, O'Connor EP, Jeong SH, Leeson R, Barry RM, Kramkowski JF, Zhang Z, Polacek L, Lohr JG, Schleicher M, Lipscomb E, Saltzman A, Oliver NM, Marini L, Waks AG, Harshman LC, Tolaney SM, Van Allen EM, Winer EP, Lin NU, Nakabayashi M, Taplin ME, Johannessen CM, Garraway LA, Golub TR, Boehm JS, Wagle N, Getz G, Love JC, Meyerson M. Scalable whole-exome sequencing of cell-free DNA reveals high concordance with metastatic tumors. Nat Commun. 2017 Nov 6;8(1):1324. doi: 10.1038/s41467-017-00965-y.

Wang YA, Wu D, Auclair JR, Salisbury JP, Sarin R, Tang Y, Mozdzierz NJ, Shah K, Zhang AF, Wu SL, Agar JN, Love JC, Love KR, Hancock WS. Integrated bottom-up and top-down liquid chromatography-mass spectrometry (LC-MS) for characterization of recombinant human growth hormone degradation productsAnal Chem. 2017 Nov 3. doi: 10.1021/acs.analchem.7b03026.

Wang YA, Wu D, Auclair JR, Salisbury JP, Sarin R, Tang Y, Mozdzierz NJ, Shah K, Zhang AF, Wu SL, Agar JN, Love JC, Love KR, Hancock WS. Integrated bottom-up and top-down liquid chromatography-mass spectrometry (LC-MS) for characterization of recombinant human growth hormone degradation productsAnal Chem. 2017 Nov 3. doi: 10.1021/acs.analchem.7b03026.

Jia B, McNeil LK, Dupont CD, Tsioris K, Barry RM, Scully IL, Ogunniyi AO, Gonzalez C, Pride MW, Gierahn TM, Liberator PA, Jansen KU, Love JC. Longitudinal multiparameter single-cell analysis of macaques immunized with pneumococcal protein-conjugated or unconjugated polysaccharide vaccines reveals distinct antigen specific memory B cell repertoires. PLoS One. 2017 Sep 14;12(9):e0183738. doi: 10.1371/journal.pone.0183738.

Goods BA, Hernandez AL, Lowther DE, Lucca LE, Lerner BA, Gunel M, Raddassi K, Coric V, Hafler DA, Love JC. Functional differences between PD-1+ and PD-1- CD4+ effector T cells in healthy donors and patients with glioblastoma multiforme. PLoS One. 2017 Sep 7;12(9):e0181538. doi: 10.1371/journal.pone.0181538.

Matthews CB, Kuo A, Love KR, Love JC. Development of a general defined medium for Pichia pastorisBiotechnol Bioeng. 2017 Sep 2. doi: 10.1002/bit.26440.

Matthews CB, Wright C, Kuo A, Colant N, Westoby M, Love JC. Reexamining opportunities for therapeutic protein production in eukaryotic microorganisms. Biotechnol Bioeng. 2017 Nov;114(11):2432-2444. doi: 10.1002/bit.26378.

Severson KA, Monian B, Love JC, Braatz RD. A method for learning a sparse classifier in the presence of missing data for high-dimensional biological datasets. Bioinformatics. 2017 Sep 15;33(18):2897-2905. doi: 10.1093/bioinformatics/btx224.

Gierahn TM, Wadsworth MH 2nd, Hughes TK, Bryson BD, Butler A, Satija R, Fortune S, Love JC, Shalek AK. Seq-Well: portable, low-cost RNA sequencing of single cells at high throughputNat Methods. 2017 Feb 13. doi: 10.1038/nmeth.4179.

Taylor RA, Chang CF, Goods BA, Hammond MD, Mac Grory B, Ai Y, Steinschneider AF, Renfroe SC, Askenase MH, McCullough LD, Kasner SE, Mullen MT, Hafler DA, Love JC, Sansing LH. TGF-β1 modulates microglial phenotype and promotes recovery after intracerebral hemorrhageJ Clin Invest. 2017 Jan 3;127(1):280-292. doi: 10.1172/JCI88647.


Huang Y, Ferrari G, Alter G, Forthal DN, Kappes JC, Lewis GK, Love JC, Borate B, Harris L, Greene K, Gao H, Phan TB, Landucci G, Goods BA, Dowell KG, Cheng HD, Bailey-Kellogg C, Montefiori DC, Ackerman ME. Diversity of Antiviral IgG Effector Activities Observed in HIV-Infected and Vaccinated SubjectsJ Immunol. 2016 Dec 15;197(12):4603-4612.

Chen X, Love JC, Navin NE, Pachter L, Stubbington MJ, Svensson V, Sweedler JV, Teichmann SA. Single-cell analysis at the thresholdNat Biotechnol. 2016 Nov 8;34(11):1111-1118. doi: 10.1038/nbt.3721.

Saung MT, Sharei A, Adalsteinsson VA, Cho N, Kamath T, Ruiz C, Kirkpatrick J, Patel N, Mino-Kenudson M, Thayer SP, Langer R, Jensen KF, Liss AS, Love JC. A Size-Selective Intracellular Delivery PlatformSmall. 2016 Sep 4. doi: 10.1002/smll.201601155.

Love KR, Shah KA, Whittaker CA, Wu J, Bartlett MC, Ma D, Leeson RL, Priest M, Borowsky J, Young SK, Love JC. Comparative genomics and transcriptomics of Pichia pastorisBMC Genomics. 2016 Aug 5;17:550. doi: 10.1186/s12864-016-2876-y. Erratum in: BMC Genomics. 2016;17(1):762.

Donius LR, Cheng Y, Choi J, Sun ZY, Hanson M, Zhang M, Gierahn TM, Marquez S, Uduman M, Kleinstein SH, Irvine D, Love JC, Reinherz EL, Kim M. Generation of Long-Lived Bone Marrow Plasma Cells Secreting Antibodies Specific for the HIV-1 gp41 Membrane-Proximal External Region in the Absence of PolyreactivityJ Virol. 2016 Sep 12;90(19):8875-90. doi: 10.1128/JVI.01089-16.

Lowther DE, Goods BA, Lucca LE, Lerner BA, Raddassi K, van Dijk D, Hernandez AL, Duan X, Gunel M, Coric V, Krishnaswamy S, Love JC, Hafler DA. PD-1 marks dysfunctional regulatory T cells in malignant gliomasJCI Insight. 2016 Apr 21;1(5). pii: e85935.

Liao MC, Muratore CR, Gierahn TM, Sullivan SE, Srikanth P, De Jager PL, Love JC, Young-Pearse TL. Single-Cell Detection of Secreted Aβ and sAPPα from Human IPSC-Derived Neurons and AstrocytesJ Neurosci. 2016 Feb 3;36(5):1730-46. doi: 10.1523/JNEUROSCI.2735-15.2016.


Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencingTsioris K, Gupta NT, Ogunniyi AO, Zimnisky RM, Qian F, Yao Y, Wang X, Stern JN, Chari R, Briggs AW, Clouser CR, Vigneault F, Church GM, Garcia MN, Murray KO, Montgomery RR, Kleinstein SH, Love JC. Integr Biol (Camb). 2015 Dec;7(12):1587-97. doi: 10.1039/c5ib00169b.

Tsioris K, Gupta NT, Ogunniyi AO, Zimnisky RM, Qian F, Yao Y, Wang X, Stern JN, Chari R, Briggs AW, Clouser CR, Vigneault F, Church GM, Garcia MN, Murray KO, Montgomery RR, Kleinstein SH, Love JC. Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing. Integr Biol (Camb) 2015. 2015 Dec;7(12):1587-97. doi: 10.1039/c5ib00169b.

Patil SU, Ogunniyi AO, Calatroni A, Tadigotla VR, Ruiter B, Ma A, Moon J, Love JC, Shreffler WG. Peanut oral immunotherapy transiently expands circulating Ara h2-specific B cells with a homologous repertoire in unrelated subjects. J Allergy Clin Immunol. 2015 Jul;136(1):125-134.e12. doi: 10.1016/j.jaci.2015.03.026.

Mozdzierz NJ, Love KR, Lee KS, Lee HL, Shah KA, Ram RJ, Love JC. A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production. Lab Chip. 2015 Jun 30;15(14):2918-22. doi: 10.1039/c5lc00443h

Ozkumur AY, Goods BA, Love JC. Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning. Small. 2015 Jun 29. doi: 10.1002/smll.201500674.

Shah KA, Clark JJ, Goods BA, Politano TJ, Mozdzierz NJ, Zimnisky RM, Leeson RL, Love JC, Love KR. Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris. Biotechnol Bioeng. 2015 Jun 1. doi: 10.1002/bit.25663.

Cao Y, Goods BA, Raddassi K, Nepom GT, Kwok WW, Love JC, Hafler DA. Functional inflammatory profiles distinguish myelin-reactive T cells from patients with
multiple sclerosis
. Sci Transl Med. 2015 May 13;7(287):287ra74. doi: 10.1126/scitranslmed.aaa8038.

Zhang CZ, Adalsteinsson VA, Francis J, Cornils H, Jung J, Maire C, Ligon KL, Meyerson M, Love JC. Calibrating genomic and allelic coverage bias in single-cell
. Nat Commun. 2015 Apr 16;6:6822. doi: 10.1038/ncomms7822.


Torres AJ, Hill AS, Love JC. Nanowell-based immunoassays for measuring single-cell secretion: characterization of transport and surface binding. Anal Chem. 2014 Dec 2;86(23):11562-9. doi: 10.1021/ac4030297. Epub 2014 Nov 17.

Yao X, Williamson C, Adalsteinsson VA, D'Agostino RS, Fitton T, Smaroff GG, William RT, Wittrup KD, Love JC. Tumor cells are dislodged into the pulmonary vein during lobectomy. J Thorac Cardiovasc Surg. 2014 Dec;148(6):3224-3231.e5. doi: 10.1016/j.jtcvs.2014.06.074. Epub 2014 Jul 23.

Castellarnau M, Szeto GL, Su HW, Tokatlian T, Love JC, Irvine DJ, Voldman J. Stochastic Particle Barcoding for Single-Cell Tracking and Multiparametric Analysis. Small. 2014 Sep 2. doi: 10.1002/smll.201401369. 

Tsioris K, Torres AJ, Douce TB, Love JC. A new toolbox for assessing single cells. Annu Rev Chem Biomol Eng. 2014;5:455-77. doi: 10.1146/annurev-chembioeng-060713-035958.

Francis JM, Zhang CZ, Maire CL, Jung J, Manzo VE, Adalsteinsson VA, Homer H, Haidar S, Blumenstiel B, Pedamallu CS, Ligon AH, Love JC, Meyerson M, Ligon KL. EGFR variant heterogeneity in glioblastoma resolved through single-nucleus sequencing. Cancer Discov. 2014 Jun 3. pii: CD-13-0879.

Adalsteinsson VA, Love JC. Towards Engineered Processes for Sequencing-Based Analysis of Single Circulating Tumor Cells. Curr Opin Chem Eng. 2014 May 1;4:97-104.

Lohr JG, Adalsteinsson VA, Cibulskis K, Choudhury AD, Rosenberg M, Cruz-Gordillo P, Francis JM, Zhang CZ, Shalek AK, Satija R, Trombetta JJ, Lu D, Tallapragada N, Tahirova N, Kim S, Blumenstiel B, Sougnez C, Lowe A, Wong B, Auclair D, Van Allen EM, Nakabayashi M, Lis RT, Lee GS, Li T, Chabot MS, Ly A, Taplin ME, Clancy TE, Loda M, Regev A, Meyerson M, Hahn WC, Kantoff PW, Golub TR, Getz G, Boehm JS, Love JC. Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer. Nat Biotechnol. 2014 May;32(5):479-84. doi: 10.1038/nbt.2892. Epub 2014 Apr 20.

Ogunniyi AO, Thomas BA, Politano TJ, Varadarajan N, Landais E, Poignard P, Walker BD,Kwon DS, Love JC. Profiling human antibody responses by integrated single-cell analysis. Vaccine. 2014 May 19;32(24):2866-73. doi: 10.1016/j.vaccine.2014.02.020. Epub 2014 Mar 3

Yao X, Choudhury AD, Yamanaka YJ, Adalsteinsson VA, Gierahn TM, Williamson CA, Lamb CR, Taplin ME, Nakabayashi M, Chabot MS, Li T, Lee GS, Boehm JS, Kantoff PW, Hahn WC, Wittrup KD, Love JC. Functional analysis of single cells identifies a rare subset of circulating tumor cells with malignant traits. Integr Biol (Camb). 2014 Apr;6(4):388-98. doi: 10.1039/c3ib40264a. Epub 2014 Feb 13.

Zhou P, Shaffer DR, Alvarez Arias DA, Nakazaki Y, Pos W, Torres AJ, Cremasco V, Dougan SK, Cowley GS, Elpek K, Brogdon J, Lamb J, Turley SJ, Ploegh HL, Root DE, Love JC, Dranoff G, Hacohen N, Cantor H, Wucherpfennig KW. In vivo discovery of immunotherapy targets in the tumour microenvironment. Nature. 2014 Feb 6;506(7486):52-7. doi: 10.1038/nature12988. Epub 2014 Jan 29.

Gierahn TM, Loginov D, Love JC. Crossword: a fully automated algorithm for the segmentation and quality control of protein microarray images. J Proteome Res. 2014 Feb 7;13(2):362-71. doi: 10.1021/pr401167h. Epub 2014 Jan 24.



Adalsteinsson, V. A., Tahirova, N., Tallapragada, N., Yao, X., Campion, L., Angelini, A., Douce, T. B., Huang, C., Bowman, B., Williamson, C. A., Kwon, D. S., Wittrup, K. D. & Love, J. C. Single cells from human primary colorectal tumors exhibit polyfunctional heterogeneity in secretions of ELR+ CXC chemokines. Integr Biol (Camb) 5, 1272-1281, doi:10.1039/c3ib40059j (2013).

Reuel, N. F., Grassbaugh, B., Kruss, S., Mundy, J. Z., Opel, C., Ogunniyi, A. O., Egodage, K., Wahl, R., Helk, B., Zhang, J., Kalcioglu, Z. I., Tvrdy, K., Bellisario, D. O., Mu, B., Blake, S. S., Van Vliet, K. J., Love, J. C., Wittrup, K. D. & Strano, M. S. Emergent Properties of Nanosensor Arrays: Applications for Monitoring IgG Affinity Distributions, Weakly Affined Hypermannosylation, and Colony Selection for Biomanufacturing. ACS Nano, doi:10.1021/nn403215e (2013).

Nguyen, C. Q., Ogunniyi, A. O., Karabiyik, A. & Love, J. C. Single-cell analysis reveals isotype-specific autoreactive B cell repertoires in Sjogren's syndrome. PLoS One 8, e58127, doi:10.1371/journal.pone.0058127 (2013).

Love, K. R., Bagh, S., Choi, J. & Love, J. C. Microtools for single-cell analysis in biopharmaceutical development and manufacturing. Trends Biotechnol 31, 280-286, doi:10.1016/j.tibtech.2013.03.001 (2013).

Torres, A. J., Contento, R. L., Gordo, S., Wucherpfennig, K. W. & Love, J. C. Functional single-cell analysis of T-cell activation by supported lipid bilayer-tethered ligands on arrays of nanowells. Lab Chip 13, 90-99, doi:10.1039/c2lc40869d (2013).

Love, J. C., Love, K. R. & Barone, P. W. Enabling Global Access to High-Quality Biopharmaceuticals. Current Opinion in Chemical Engineering (2013).


Yamanaka, Y. J., Szeto, G. L., Gierahn, T. M., Forcier, T. L., Benedict, K. F., Brefo, M. S., Lauffenburger, D. A., Irvine, D. J. & Love, J. C. Cellular barcodes for efficiently profiling single-cell secretory responses by microengraving. Anal Chem 84, 10531-10536, doi:10.1021/ac302264q (2012).

Yamanaka, Y. J., Gierahn, T. M. & Love, J. C. The dynamic lives of T cells: new approaches and themes. Trends Immunol., doi:10.1016/ (2012).

Yamanaka, Y. J., Berger, C. T., Sips, M., Cheney, P. C., Alter, G. & Love, J. C. Single-cell analysis of the dynamics and functional outcomes of interactions between human natural killer cells and target cells. Integr Biol (Camb) 4, 1175-1184, doi:10.1039/c2ib20167d (2012).

Yalcin, A., Yamanaka, Y. J. & Love, J. C. Analytical technologies for integrated single-cell analysis of human immune responses. Methods Mol Biol 853, 211-235, doi:10.1007/978-1-61779-567-1_16 (2012).

Love, K. R., Politano, T. J., Panagiotou, V., Jiang, B., Stadheim, T. A. & Love, J. C. Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity. PLoS One 7, e37915, doi:10.1371/journal.pone.0037915 (2012).

Varadarajan, N., Kwon, D. S., Law, K. M., Ogunniyi, A. O., Anahtar, M. N., Richter, J. M., Walker, B. D. & Love, J. C. Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving. Proc. Natl. Acad. Sci. USA (2012).

Han, Q., Bagheri, N., Bradshaw, E. M., Hafler, D. A., Lauffenburger, D. A. & Love, J. C. Polyfunctional responses by human T cells result from sequential release of cytokines. Proc. Natl. Acad. Sci. USA 109, 1605-1612, doi:10.1073/pnas.1117194109 (2012).
(See also accompanying commentary:  Haining, W.N., Travels in time:  Assessing the functional complexity of T cells. Proc. Natl. Acad. Sci. USA 109, 1359-1360, doi:10.1073/pnas.1119856109 (2012).)



Varadarajan, N., Julg, B., Yamanaka, Y. J., Chen, H., Ogunniyi, A. O., McAndrew, E., Porter, L. C., Piechocka-Trocha, A., Hill, B. J., Douek, D. C., Pereyra, F., Walker, B. D. & Love, J. C. A high-throughput single-cell analysis of human CD8 T cell functions reveals discordance for cytokine secretion and cytolysis. J. Clin. Invest. 121, 4322-4331, doi:10.1172/JCI58653 (2011).

Zhao, W., Schafer, S., Choi, J., Yamanaka, Y. J., Lombardi, M. L., Bose, S., Carlson, A. L., Phillips, J. A., Teo, W., Droujinine, I. A., Cui, C. H., Jain, R. K., Lammerding, J., Love, J. C., Lin, C. P., Sarkar, D., Karnik, R. & Karp, J. M. Cell-surface sensors for real-time probing of cellular environments. Nat. Nanotechnol. 6, 524-531, doi:10.1038/nnano.2011.101 (2011).

Choi, J., Love, K. R., Gong, Y., Gierahn, T. M. & Love, J. C. Immuno-hybridization chain reaction for enhancing detection of individual cytokine-secreting human peripheral mononuclear cells. Anal. Chem. 83, 6890-6895, doi:10.1021/ac2013916 (2011).

Panagiotou, V., Love, K. R., Jiang, B., Nett, J., Stadheim, T. & Love, J. C. Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving. Appl. Environ. Microb. 77, 3154-3156, doi:10.1128/AEM.00104-11 (2011).



Love, J. C. Making antibodies from scratch. Nat. Biotechnol. 28, 1176-1178, doi:10.1038/nbt1110-1176 (2010).

Love, J.C. Integrated process design for single-cell analytical technologies. AIChE J. 56, 2496-2502 (2010).

Gong, Y., Ogunniyi, A.O. & Love, J.C. Massively parallel detection of gene expression in single cells using subnanoliter wells. Lab Chip 10, 2334-2337 (2010).

Han, Q., Bradshaw, E.M., Nilsson, B., Hafler, D.A. & Love, J.C. Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving. Lab Chip 10, 1391-1400 (2010). 

Love, K.R., Panagiotou, V., Jiang, B., Stadheim, T.A. & Love, J.C. Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. Biotechnol. Bioeng. 106, 319-325 (2010).

Choi, J.H., Ogunniyi, A.O., Du, M., Du, M., Kretschmann, M., Eberhardt, J. & Love, J.C. Development and optimization of a process for automated recovery of single cells identified by microengraving. Biotechnol. Prog. 26, 888-895 (2010).

Schmidt, D.J., Pridgen, E.M., Hammond, P.T. & Love, J.C. Layer-by-layer assembly of a pH-sensitive and electrochromic thin film. J. Chem. Educ. 87, 208-211 (2010).

Song, Q., Han, Q., Bradshaw, E.M., Kent, S.C., Raddassi, K., Nilsson, B., Nepom, G.T., Hafler, D.A. & Love, J.C. On-chip activation and subsequent detection of individual antigen-specific T cells. Anal. Chem. 82, 473-477 (2010).



Ogunniyi, A.O., Story, C.M., Papa, E., Guillen, E. & Love, J.C. Screening individual hybridomas by microengraving to discover monoclonal antibodies. Nature Protocols 4, 767-782 (2009).

Hu, C.-C.A., Dougan, S.K., McGehee, A.M., Love, J.C. & Ploegh, H.L. XBP-1 regulates signal transduction, transcription factors and bone marrow colonization in B cells. EMBO J. 28, 1624-1636 (2009).

Ronan, J.L., Story, C.M., Papa, E. & Love, J.C. Optimization of the surfaces used to capture antibodies from single hybridomas reduces the time required for microengraving. J. Immunol. Methods 340, 164-169 (2009).



Story, C.M., Papa, E., Hu, C.-C.A., Ronan, J.L., Herlihy, K., Ploegh, H.L. & Love, J.C. Profiling antibody responses by multiparametric analysis of single B cells. Proc. Natl. Acad. Sci. U. S. A. 105, 17902-17907 (2008).

Bradshaw, E. M., Kent, S. C., Tripuraneni, V., Orban, T., Ploegh, H. L., Hafler, D. A. & Love, J. C. Concurrent detection of secreted products from human lymphocytes by microengraving: cytokines and antigen-reactive antibodies. Clin Immunol 129, 10-18, doi:10.1016/j.clim.2008.06.009 (2008).

Wong, A. P., Castillejos, R. P., Love, J. C. & Whitesides, G. M. Partitioning Microfluidic Channels with Hydrogel to Construct Tunable 3-D Cellular Microenvironments. Biomaterials 29, 1853-1861 (2008).



Whitesides, G. M. & Love, J. C. The Art of Building Small. Scientific American Reports (2007).

Leduc, P. R., Wong, M. S., Ferreira, P. M., Groff, R. E., Haslinger, K., Koonce, M. P., Lee, W. Y., Love, J. C., McCammon, J. A., Monteiro-Riviere, N. A., Rotello, V. M., Rubloff, G. W., Westervelt, R. & Yoda, M. Towards an in vivo biologically inspired nanofactory. Nature Nanotechnology 2, 3-7 (2007).

Vyas, J. M., Kim, Y.-M., Artavanis-Tsakonas, K., Love, J. C., van der Veen, A. G. & Ploegh, H. L. Tubulation of Class II MHC compartments is microtubule-dependent and involves multiple endolysosomal membrane proteins in primary dendritic cells. J. Immunol. 178, 7199-7210 (2007).

Leduc, P. R., Wong, M. S., Ferreira, P. M., Groff, R. E., Haslinger, K., Koonce, M. P., Lee, W. Y., Love, J. C., McCammon, J. A., Monteiro-Riviere, N. A., Rotello, V. M., Rubloff, G. W., Westervelt, R. & Yoda, M. Towards an in vivo biologically inspired nanofactory. Nature Nanotechnology 2, 3-7 (2007).